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The nonparametric estimators of species richness are some of the most widely used extrapolation methods in studies of biodiversity. These studies need relative large samples to achieve total diversity (including rare species), and estimators are routinely used to alleviate this problem. In the existing literature, parasite communities have received little attention. In fact, the present study is the first dealing with parasites in invertebrate hosts. Real data on seasonal diversity of larval digeneans in the snails Heleobia conexa and Heleobia australis from Mar Chiquita coastal lagoon (Argentina) were used to evaluate seven nonparametric estimators of species richness, observing the behavior of each method and its ability to estimate the richness using different subsample sizes. In addition, estimated species richness was compared with the previously observed species richness in the parasite component …