作者
Faisal Ali Anwarali Khan, Awang Ahmad Sallehin Awang Husaini
发表日期
2006
期刊
Biotechnology
卷号
5
期号
3
页码范围
391-403
简介
The effect of Solid State Fermentation (SSF) parameters on the production of extracellular c-amylase and cellulase (endoglucanases) by Bacillus amyloliquefaciens UMAS 1002 using sago pith residue hampas in shaker flasks was investigated in this study. The appropriate incubation period, temperature, pH, agitation speed, inoculum concentration, hampas concentration and additive substrate effect were optimized for enzyme production. The activity of c-amylase and cellulase was at 14.19 and 13.15 IU ml. T", respectively in optimal culture medium. Maximum yield for both enzymes were achieved by employing 4% w/v hampas in 0, 2 M citrate buffer at pH 6 and incubated at 40 C for 6 h with agitation speed of 100 rpm. Inoculum concentrations were found to be optimum at 3% v/v and 4% v/v for c-amylase and cellulase, respectively. Enzyme activity was 2.8 (1080 IU mLT") and 3.2 (938 IU mLT") fold higher for c …
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