作者
Shaobo Ruan, Wellington J Rody Jr, Shivani S Patel, Lina I Hammadi, Macey L Martin, Lorraine P de Faria, George Daaboul, Leif S Anderson, Mei He, L Shannon Holliday
发表日期
2023
期刊
Extracellular vesicles and circulating nucleic acids
卷号
4
期号
3
页码范围
518
出版商
NIH Public Access
简介
Aim:
Receptor activator of nuclear factor-kappa B (RANK)-containing extracellular vesicles (EVs) bind RANK-Ligand (RANKL) on osteoblasts, and thereby simultaneously inhibit bone resorption and promote bone formation. Because of this, they are attractive candidates for therapeutic bone anabolic agents. Previously, RANK was detected in 1 in every 36 EVs from osteoclasts by immunogold electron microscopy. Here, we have sought to characterize the subpopulation of EVs from osteoclasts that contains RANK in more detail.
Methods:
The tetraspanins CD9 and CD81 were localized in osteoclasts by immunofluorescence. EVs were visualized by transmission electron microscopy. A Single Particle Interferometric Reflectance Imaging Sensor (SP-IRIS) and immunoaffinity isolations examined whether RANK is enriched in specific types of EVs.
Results:
Immunofluorescence showed CD9 was mostly on or near the …
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S Ruan, WJ Rody Jr, SS Patel, LI Hammadi, ML Martin… - Extracellular vesicles and circulating nucleic acids, 2023
