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A comparative study of food selectivity of the benthic copepod Tigriopus japonicus and the pelagic copepod Paracyclopina nana was performed by feeding four different microalgae (Tetraselmis suecica, Isochrysis galbana, Chlorella sp., and Nannochloropsis oculata). To examine whether the morphological characteristics of the microalga could influence the food selectivity, we investigated the movement, color, and size of the microalgae. The result indicated significant differences only in the movement of microalga. However, no significant differences in most of the microalgae were observed in feeding rate determination in 24 h, indicating plenty amounts of microalgae were administered to both copepods. In the T. suecica- and I. galbana-fed groups, no significant nitrogen isotopic turnover was observed, indicating both microalgae might be suitable for experimental copepods. However, the difference in the …