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Two simple and rapid DNA extraction procedures based on Chelex resin were compared and optimized to get reliable amplification by PCR from diverse sources of fish tissue (fin, liver, muscle, blood, scales and individual eggs) at numerous nuclear loci (13 microsatellites and 4 lacZ transgenes) and mitochondrial loci (ND5/6 and cytochrome b/D loop). The 1st procedure worked for most of the markers under investigation. However, it did not result in satisfactory PCR products for 6 microsatellite loci and for 1 mitochondrial DNA domain. This problem was addressed with the 2nd procedure by adding proteinase K from the extraction start. This pretreatment provided an all-or-none improvement, as it had a strong positive effect on 'refractory' loci (much better PCR yield and reduced number of unspecific and super numerous bands) but did not quantitatively enhance or attenuate the amplification of all other loci.