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Human herpesvirus 8 (HHV‐8) infection is common in sub‐Saharan Africa, but its prevalence in Mozambique is unknown. The seroprevalence of HHV‐8 in a cohort of individuals seen at public health centers in Northern (n = 208), Central (n = 226), or Southern (n = 318) Mozambique was examined. All individuals were interviewed to obtain socioeconomic, demographic and clinical data and were tested for serum anti‐HHV‐8 antibodies using an immunofluorescence assay. The overall frequency of HHV‐8 antibodies was 21.4% and, in spite of the diversity of epidemiological characteristics of the tested individuals, did not differ significantly among regions: 18.7%, 24.3% and 21.4% in the North, Center, and South, respectively (χ², 2.37; P = 0.305). The variables that were associated significantly with the presence of HHV‐8 antibodies were gender, age, level of education, number of siblings and HIV …