作者
Teresina Laragione, Max Brenner, Adriana Mello, Marc Symons, Pércio S Gulko
发表日期
2008/8
期刊
Arthritis & Rheumatism: Official Journal of the American College of Rheumatology
卷号
58
期号
8
页码范围
2296-2306
出版商
Wiley Subscription Services, Inc., A Wiley Company
简介
Objective
The synovial fibroblast, or fibroblast‐like synoviocyte (FLS), has a central role in pannus invasion and destruction of cartilage and bone in rheumatoid arthritis (RA). However, regulation of the FLS remains incompletely understood. The aim of this study was to determine whether the invasive properties of FLS are genetically regulated by arthritis severity loci.
Methods
DA rats (arthritis susceptible) and rat strains congenic for arthritis‐protective intervals were studied. Primary FLS cell lines were generated from each strain and used in a well‐established FLS invasion model through a collagen‐rich barrier. Cells or culture supernatants were analyzed for gene expression, activity of different matrix metalloproteinases (MMPs), cytoskeleton integrity, and cell proliferation.
Results
The median number of FLS from DA.F344(Cia5d) rats that invaded through the collagen‐rich barrier was reduced 86.5% compared …
学术搜索中的文章
T Laragione, M Brenner, A Mello, M Symons, PS Gulko - Arthritis & Rheumatism: Official Journal of the American …, 2008