作者
Dieter Jendrossek, Ingrid Knoke, Rahim Bahodjb Habibian, Alexander Steinbüchel, Hans Günter Schlegel
发表日期
1993/1
期刊
Journal of environmental polymer degradation
卷号
1
页码范围
53-63
出版商
Kluwer Academic Publishers-Plenum Publishers
简介
Bacteria capable of growing on poly(3-hydroxybutyrate), PHB, as the sole source of carbon and energy were isolated from various soils, lake water, activated sludge, and air. Although all bacteria utilized a wide variety of monomeric substrates for growth, most of the strains were restricted to degrade PHB and copolymers of 3-hydroxybutyrate and 3-hydroxyvalerate, P(3HB-co-3HV). Five strains were also able to decompose a homopolymer of 3-hydroxyvalerate, PHV. Poly(3-hydroxyoctanoate), PHO, was not degraded by any of the isolates. One strain, which was identified asComamonas sp., was selected, and the extracellular depolymerase of this strain was purified from the medium by ammonium sulfate precipitation and by chromatography on DEAE-Sephacel and Butyl-Sepharose 4B. The purified PHB depolymerase was not a glycoprotein. The relative molecular masses of the native enzyme and of the …
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D Jendrossek, I Knoke, RB Habibian, A Steinbüchel… - Journal of environmental polymer degradation, 1993