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Yellows‐diseased plants of Crepissetosa (hawksbeard), Knautiaarvensis (field scabious), Convolvulusarvensis (field bindweed), Picrisechioides (bristly oxtongue), Echiumvulgare (blueweed) and Calendulaofficinalis (pot marigold) collected in central and southern Italy were examined for phytoplasma infection by means of polymerase chain reaction (PCR) technology using universal phytoplasma primers directed to ribosomal sequences. The detected phytoplasmas were characterized and differentiated using restriction fragment length polymorphism analysis of PCR‐amplified DNA. The phytoplasma detected in diseased pot marigold plants was identified as a member of the aster yellows group and proved indistinguishable from a strain of the American aster yellows phytoplasma. The phytoplasma identified in diseased field bindweed plants is a putative new type of the stolbur group that differed from the typical …