作者
Eric Londin, Phillipe Loher, Aristeidis G Telonis, Kevin Quann, Peter Clark, Yi Jing, Eleftheria Hatzimichael, Yohei Kirino, Shozo Honda, Michelle Lally, Bharat Ramratnam, Clay ES Comstock, Karen E Knudsen, Leonard Gomella, George L Spaeth, Lisa Hark, L Jay Katz, Agnieszka Witkiewicz, Abdolmohamad Rostami, Sergio A Jimenez, Michael A Hollingsworth, Jen Jen Yeh, Chad A Shaw, Steven E McKenzie, Paul Bray, Peter T Nelson, Simona Zupo, Katrien Van Roosbroeck, Michael J Keating, George A Calin, Charles Yeo, Masaya Jimbo, Joseph Cozzitorto, Jonathan R Brody, Kathleen Delgrosso, John S Mattick, Paolo Fortina, Isidore Rigoutsos
发表日期
2015/3/10
期刊
Proceedings of the National Academy of Sciences
卷号
112
期号
10
页码范围
E1106-E1115
出版商
National Academy of Sciences
简介
Two decades after the discovery of the first animal microRNA (miRNA), the number of miRNAs in animal genomes remains a vexing question. Here, we report findings from analyzing 1,323 short RNA sequencing samples (RNA-seq) from 13 different human tissue types. Using stringent thresholding criteria, we identified 3,707 statistically significant novel mature miRNAs at a false discovery rate of ≤0.05 arising from 3,494 novel precursors; 91.5% of these novel miRNAs were identified independently in 10 or more of the processed samples. Analysis of these novel miRNAs revealed tissue-specific dependencies and a commensurate low Jaccard similarity index in intertissue comparisons. Of these novel miRNAs, 1,657 (45%) were identified in 43 datasets that were generated by cross-linking followed by Argonaute immunoprecipitation and sequencing (Ago CLIP-seq) and represented 3 of the 13 tissues, indicating …
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