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With the continuous increase in the population and the industrial sector, it has been observed that there has been a subjugation of the sources of energy along with the reduction in the repositories of fuel. Lignin is one such potential candidate to curb the issues associated with the environment along with the replacement of reservoirs of fuel based on petroleum. Lignin is extremely resistant to degradation processes which usually pass via unmodified ruminants. All together with cellulose and hemicelluloses, lignin is an important component in the arrangements of the cell wall at a minute scale and hence the formation of structures made up of lignin–carbohydrate network. Certain problems such as complex molecular structures of lignin result in difficultly during the isolation and identification approaches and hence significant issues while developing bio-refineries based on the biomass.