作者
Lorraine C Racusen, C Monteil, Anita Sgrignoli, Margit Lucskay, S Marouillat, John GS Rhim, Jean-paul Morin
发表日期
1997/3/1
期刊
Journal of Laboratory and Clinical Medicine
卷号
129
期号
3
页码范围
318-329
出版商
Mosby
简介
Few model systems exist for the study of injury to human renal proximal tubule epithelium. Optimized differentiated human renal epithelial cell lines with extended in vitro growth potential would provide an alternative model system to primary culture or other available non-human mammalian kidney cell lines. For this purpose, human renal tubule epithelial cells were isolated from normal kidney cortex and exposed in culture to a hybrid immortalizing virus, adenovirus 12-SV40. Cell lines were developed by limiting dilution, and three selected cell lines were screened for growth pattern, production of immortalizing virus, tumorigenicity, and ploidy. Cell lines were also monitored for response to inducer agents and matrix factors and were screened for expression of biochemical properties and differentiation markers of renal epithelium. All three are nonproducers of the immortalizing virus and are nontumorigenic. They …
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LC Racusen, C Monteil, A Sgrignoli, M Lucskay… - Journal of Laboratory and Clinical Medicine, 1997