作者
J-P Morin, F Fouquet, C Monteil, E Le Prieur, E Vaz, F Dionnet
发表日期
1999/6
期刊
Cell biology and toxicology
卷号
15
页码范围
143-152
出版商
Kluwer Academic Publishers
简介
The purpose of this study was the development of a new incubation system that can allow continuous exposure of lung tissue to complex atmospheres as a tool for the assessment of aerial environmental lung toxicology. To assess the pertinence of this new exposure system, we studied the impact of diesel engine exhausts as a complex atmosphere containing both gaseous and particulate fractions and have been able to discriminate between the toxicological impacts of the gaseous phase and particulate matter from diesel exhausts. Continuous flow-through rotating chambers with controlled pO2, pCO2, and hygrometry have been designed in which lung slices are positioned in rolling inserts that allow free access of atmosphere to the exposed lung tissue. Under control conditions, cell viability was preserved for at least 48 h as assessed by intracellular ATP, GSH, and K+ levels and slice O2 consumption …
引用总数
学术搜索中的文章
JP Morin, F Fouquet, C Monteil, E Le Prieur, E Vaz… - Cell biology and toxicology, 1999