作者
Piero Marchetti, Marco Bugliani, R Lupi, Lorella Marselli, Mathilde Masini, Ugo Boggi, Franco Filipponi, GC Weir, Decio L Eizirik, Miriam Cnop
发表日期
2007/12
期刊
Diabetologia
卷号
50
页码范围
2486-2494
出版商
Springer-Verlag
简介
Aims/hypothesis
Pancreatic beta cells have highly developed endoplasmic reticulum (ER) due to their role in insulin secretion. Since ER stress has been associated with beta cell dysfunction, we studied several features of beta cell ER in human type 2 diabetes.
Methods
Pancreatic samples and/or isolated islets from non-diabetic controls (ND) and type 2 diabetes patients were evaluated for insulin secretion, apoptosis (electron microscopy and ELISA), morphometric ER assessment (electron microscopy), and expression of ER stress markers in beta cell prepared by laser capture microdissection and in isolated islets.
Results
Insulin release was lower and beta cell apoptosis higher in type 2 diabetes than ND islets. ER density volume was significantly increased in type 2 diabetes beta cells. Expression of alpha …
引用总数
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P Marchetti, M Bugliani, R Lupi, L Marselli, M Masini… - Diabetologia, 2007