作者
Min-Yuan Chia, Chun-Yang Lin, Yi-Qi Yeh, U-Ser Jeng, Wen-Guey Wu, Min-Shi Lee
发表日期
2019/3/22
期刊
Vaccine
卷号
37
期号
13
页码范围
1897-1903
出版商
Elsevier
简介
Human infections of novel avian influenza A virus (H7N9) emerged in early 2013 and caused about 40% case-fatality through 2017. Therefore, development of influenza H7N9 vaccines is critical for pandemic preparedness. Currently, there are three means of production of commercial influenza vaccines: egg-based, mammalian cell-based, and insect cell-based platforms. The insect cell-based platform has the advantage of high speed in producing recombinant protein. In this study, we evaluate the stability and immunogenicity of two different influenza H7 HA expression constructs generated using the baculovirus system, including membrane-based full-length HA (mH7) and secreted ectodomain-based H7 (sH7). The mH7 construct could form an oligomer-rosette structure and had a high hemagglutinin (HA) titer 8192. In contrast to mH7, the sH7 construct could not form an oligomer-rosette structure and did not …
学术搜索中的文章
MY Chia, CY Lin, YQ Yeh, US Jeng, WG Wu, MS Lee - Vaccine, 2019