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The aim of the study was to compare an antagonistic activity of Trichoderma spp. and yeasts on pathogenic Fusarium spp. in in vitro conditions. Two Trichoderma species (T. viride and T. harzianum), two yeast species (Candida albicans and Trichosporon pullulans) and five Fusarium species (F. culmorum, F. graminearum, F. poae, F. oxysporum and F. avenaceum) were tested. Fungi were inoculated in pairs on PDA plates and incubated in two temperatures: 20ºC and 26ºC. The fungi diameter was measured. T. harzianum and T. viride affected the pathogens the most and T. pullulans the least. Incubation temperature did not affect Trichoderma spp. C. albicans was more active in the higher temperature. Among pathogens the most vulnerable were F. graminearum, F. avenaceum and F. oxysporum.