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Resistant starch (RS) as a grain component characteristic, is gaining recognition because food containing higher RS are found to have lower glycemic index, which in turn provide health benefits to consumers apart from energy sources for the body. In this study, 310 indica rice accessions were selected from the 3000 rice genome sequencing project (3K RGP) based on diversity in grain quality phenotypes, similarity in flowering time and availability of high-quality reads genome sequence. This diversity panel was assayed for RS, digestible carbohydrates (DC) contents and other grain characteristics, which resulted to five (5) population clustering of the sampled rice accessions. RS grain content of the samples ranged from 0.169%-3.366%, while DC grain content ranged from 69.94%-82.59%. RS and amylose content (AC) were positively correlated at 0.44, while DC and protein content (PC) were negatively correlated at-0.51. For targeted genome association (targeted GWAS), 33 lines with contrasting grain characteristics were selected. This sub-set diversity panel represents each of the five population clusters classified based in all phenotyped grain characteristics. Genes involved in starch biosynthesis and degradation, and in synthesis and regulation of non-starch polysaccharides (NSPs) as previously reported were used in the targeted associations. Using Efficient Mixed-Model Association eXpedited (EMMAX) to do the targeted genome associations, 66 SNPs associated to rice grain RS content (p0. 01) were identified. The Waxy locus at chromosome 6, which encodes for granule bound starch synthase, had 28 accumulated SNPs. Non …