作者
Chih-Feng Yen, Sung Hoon Kim, Shuen-Kuei Liao, Cem Atabekoglu, Serpil Uckac, Aydin Arici, Sefa Arlier, Chyi-Long Lee, Hsin-Shih Wang, Umit A Kayisli
发表日期
2017/3/1
期刊
Fertility and Sterility
卷号
107
期号
3
页码范围
803-812
出版商
Elsevier
简介
Objective
To study the impact of integrin-linked kinase (ILK) in endometrial stromal cells (ESCs) during decidualization.
Design
Laboratory study with the use of human endometrium.
Setting
University hospital.
Patient(s)
Fertile reproductive-age women who had not received hormonal treatment for 3 months before tissue collection.
Intervention(s)
Endometrium tissue collection, in vitro decidualization of isolated ESCs, and small interfering (si) RNA transfection.
Main Outcome Measure(s)
Immunohistochemistry, ELISA, Western blot analysis, methylthiazolyl tetrazolium assay, and immunofluorescence staining.
Result(s)
In vivo expression of ILK is significantly increased in distended-fusiform stromal cells of late secretory endometrium and in cobblestone-shaped decidual cells of early pregnancy. During in vitro decidualization for up to 8 days, confluent cultures of isolated ESCs consistently displayed increased ILK expression …
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CF Yen, SH Kim, SK Liao, C Atabekoglu, S Uckac… - Fertility and Sterility, 2017