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Background: The presence of brown adipose tissue (BAT) in adults offers an opportunity to examine inflammatory factors that may affect metabolic function in states of obesity. Gut-derived lipopolysaccharide (LPS), which is elevated in obesity, and initiates the innate immune response in white adipose tissue, has not been fully studied in BAT. The interactions between LPS, TLR4 and ß3-adrenergic receptors in BAT is unknown. ß3-adrenergic receptor ligands as CL 316,243 (CL) induce BAT activity through UCP1-stimulation. Therefore, the objective of this study was to investigate the effect of LPS on the CL response and examine how LPS may alter mitochondrial function in BAT.

Methods: Immortalized brown adipocytes were differentiated with or without LPS (100 ng/ml, 1000 ng/ml). After treating cells with CL, RNA and protein were extracted for qRT-PCR and Western blot analysis. Mitochondrial respiration was …