作者
Lucila Okuyama Fukasawa, Cláudio Tavares Sacchi, Maria Gisele Gonçalves, Ana Paula Silva Lemos, Samanta Cristine Grassi Almeida, Adele Caterino-de-Araujo
发表日期
2021/6/1
期刊
Journal of Clinical Virology Plus
卷号
1
期号
1-2
页码范围
100012
出版商
Elsevier
简介
Introduction
Brazil is the second largest country with COVID-19 positive cases worldwide. Due to the potent spread of the virus and the scarcity of kits and supplies, the Brazilian Ministry of Health has granted authorization for the use of kits available during this emergency, without an accurate evaluation of their performance. This study compared the performance and cost-effectiveness of seven molecular assays/kits available in São Paulo, Brazil, for SARS-CoV-2 diagnosis
Materials and methods
A total of 205 nasopharyngeal/oropharyngeal samples from suspected cases of COVID-19, were tested using the following assays: (i) GeneFinder COVID-19 plus RealAmp kit; (ii) 2019-nCoV RNA PCR-Fluorescence Probing, Da An Gene Co.; (iii) in-house RT-qPCR SARS-CoV-2 IAL; (iv) 2019-nCoV kit, IDT; (v) molecular SARS-CoV-2 (E) kit, Bio-Manguinhos; (vi) Allplex 2019-nCoV modified Assay, Seegene Inc, and (vii …
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LO Fukasawa, CT Sacchi, MG Gonçalves, APS Lemos… - Journal of Clinical Virology Plus, 2021