查看文章

In order to ascertain whether erythropoietin plays a role in early and late repair processes following ischaemic skin ap injury, a dorsal, caudally based skin ap was created in rats. The rats were successively divided into four groups. Group 1 was not treated. The other groups were treated with a subcutaneous administration of 0.9% NaCl saline solution (group 2), a subcutaneous administration of vehicle (group 3) or a subcutaneous administration of 300 IU/kg/day of recombinant human erythropoietin (group 4). We evaluated the possible relationships between neutrophil accumulation, myeloperoxidase activity and content in ap tissue, ap survival, ap temperature (using telethermography) and ap revascularization (using videocapillaroscopy). Necrosis in the ap was signi® cantly less extensive in group 4 than in groups 1, 2 and 3. A signi® cant increase in neutrophil in® ltration occurred between the 1st and 24th hour in these groups, but this was not observed in group 4. These® ndings were con® rmed by biochemical data of myeloperoxidase activity and malonyldialdehyde content. Between the 1st and 7th days, we recorded an increase of about 20% in ap temperature in groups 1, 2 and 3, whereas no signi® cant variation was observed in group 4. On the 7th day, videocapillaroscopic® ndings showed an increase in the mean vascularization index in group 4. Our® ndings suggest that recombinant human erythropoietin administration can improve the wound healing process, in both early and late stages of injury, by reducing in ammatory response, increasing the density of capillaries in ischaemic aps and allowing earlier repair of a damaged area …