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The increasing market demands of β‐carotene as colorant, antioxidant and vitamin precursor, requires novel biotechnological production platforms. Yarrowia lipolytica, is an industrial organism unable to naturally synthesize carotenoids but with the ability to produce high amounts of the precursor Acetyl‐CoA. We first found that a lipid overproducer strain was capable of producing more β‐carotene than a wild type after expressing the heterologous pathway. Thereafter, we developed a combinatorial synthetic biology approach base on Golden Gate DNA assembly to screen the optimum promoter‐gene pairs for each transcriptional unit expressed. The best strain reached a production titer of 1.5 g/L and a maximum yield of 0.048 g/g of glucose in flask. β‐carotene production was further increased in controlled conditions using a fed‐batch fermentation. A total production of β‐carotene of 6.5 g/L and 90 mg/g DCW …