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To ensure the accuracy of DNA replication, to maintain genome stability, and to assure the preservation of a species, the DNA in the cell should be constantly repaired. The mismatch repair (MMR) process is crucial for gametogenesis, meiotic recombination, and the preservation of genomic integrity. Two MutS homologs (MSH4 and MSH5) and three MutL homologs (MLH1, MLH3, and PMS2) are members of the MMR family who actively participate in meiotic recombination and gametogenesis. About 50% of all cases of infertility are caused by male factors, and 15-20% of infertile males have genetic issues that result in azoospermia or severe oligozoospermia. This study aimed to find and discover the nucleotide changes in exons 7, and 8 of MLH1 gene in seven infertile and three normal fertile males through Polymerase Chain Reaction (PCR) and DNA sequencing. In this study pair of primers was designed according to the NCBI primer designing tool to amplify the exons 7, and 8 of MLH1 gene by PCR technique then sequencing the amplified product with Sanger sequencing. The sequence results aligned to NCBI database to find the possible nucleotide mismatch. Nine types of nucleotide variations have been detected of which seven of them are considered to be new recording nucleotide variations. These are the 37011581 A> T, 37011685 T> A, 37011767 C> A, 37011695 T> C, 37011579 A> T, 37011595 T> A and 37011717 T> C. However, the other two changes has been reported previously as defined in NCBI database which are 37011835 T> C with rs1553644000 T/C and 37012056 A> C with rs771612764 A/G. this stufy has found that …