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The influence of 2‐methoxyestradiol (2ME) was investigated on cell growth, morphology and spindle formation in a tumorigenic (MCF‐7) and non‐tumorigenic (MCF‐12A) epithelial breast cell line. Inhibition of cell growth was more pronounced in the MCF‐7 cells compared to the MCF‐12A cells following 2ME treatment. Dose‐dependent studies (10⁻⁵ – 10⁻⁹ M) revealed that 10⁻⁶ M 2ME inhibited cell growth by 44% in MCF‐12A cells and by 84% in MCF‐7 cells (p‐value < 0.05). 2ME‐treated MCF‐7 cells showed abnormal metaphase cells, membrane blebbing, apoptotic cells and disrupted spindle formation. These observations were either absent or less prominent in MCF‐12A cells. 2ME had no effect on the length of the cell cycle between S‐phase and the time a mitotic peak was reached in either cell line but MCF‐7 cells were blocked in mitosis with no statistically significant alterations in the …