作者
Mariana Tinajero-Trejo, Namrata Rana, Christoph Nagel, Helen E Jesse, Thomas W Smith, Lauren K Wareham, Michael Hippler, Ulrich Schatzschneider, Robert K Poole
发表日期
2016/5/10
期刊
Antioxidants & redox signaling
卷号
24
期号
14
页码范围
765-780
出版商
Mary Ann Liebert, Inc.
简介
Aims: We set out to investigate the antibacterial activity of a new Mn-based photoactivated carbon monoxide-releasing molecule (PhotoCORM, [Mn(CO)3(tpa-κ3N)]+) against an antibiotic-resistant uropathogenic strain (EC958) of Escherichia coli. Results: Activated PhotoCORM inhibits growth and decreases viability of E. coli EC958, but non-illuminated carbon monoxide-releasing molecule (CORM) is without effect. NADH-supported respiration rates are significantly decreased by activated PhotoCORM, mimicking the effect of dissolved CO gas. CO from the PhotoCORM binds to intracellular targets, namely respiratory oxidases in strain EC958 and a bacterial globin heterologously expressed in strain K-12. However, unlike previously characterized CORMs, the PhotoCORM is not significantly accumulated in cells, as deduced from the cellular manganese content. Activated PhotoCORM reacts avidly with hydrogen …
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M Tinajero-Trejo, N Rana, C Nagel, HE Jesse… - Antioxidants & redox signaling, 2016