作者
John P Rooney, Ian T Ryde, Laurie H Sanders, Evan H Howlett, Meryl D Colton, Kaylyn E Germ, Greg D Mayer, J Timothy Greenamyre, Joel N Meyer
发表日期
2015
期刊
Mitochondrial Regulation: Methods and Protocols
页码范围
23-38
出版商
Springer New York
简介
Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter, we describe methods for isolation of both mtDNA and nuclear DNA (nucDNA) and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species and cell type of the starting material and availability of specific PCR reagents.
学术搜索中的文章
JP Rooney, IT Ryde, LH Sanders, EH Howlett… - Mitochondrial Regulation: Methods and Protocols, 2015