作者
Valerio Pereno, Dario Carugo, Luca Bau, Erdinc Sezgin, Jorge Bernardino de la Serna, Christian Eggeling, Eleanor Stride
发表日期
2017/3/31
期刊
ACS omega
卷号
2
期号
3
页码范围
994-1002
出版商
American Chemical Society
简介
Giant unilamellar vesicles (GUVs) are well-established model systems for studying membrane structure and dynamics. Electroformation, also referred to as electroswelling, is one of the most prevalent methods for producing GUVs, as it enables modulation of the lipid hydration process to form relatively monodisperse, defect-free vesicles. Currently, however, it is expensive and time-consuming compared with other methods. In this study, we demonstrate that 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine GUVs can be prepared readily at a fraction of the cost on stainless steel electrodes, such as commercially available syringe needles, without any evidence of lipid oxidation or hydrolysis.
引用总数
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