作者
Rafael Mayoral, Olivia Osborn, Joanne McNelis, Andrew M Johnson, Cristina Llorente Izquierdo, Heekyung Chung, Pingping Li, Paqui G Traves, Gautam Bandyopadhyay, Ariane R Pessentheiner, Jachelle M Ofrecio, Joshua R Cook, Li Qiang, Domenico Accili, Jerrold M Olefsky
发表日期
2015/5/1
期刊
Molecular metabolism
卷号
4
期号
5
页码范围
378-391
出版商
Elsevier
简介
Objective
Adipose tissue is the primary site for lipid deposition that protects the organisms in cases of nutrient excess during obesogenic diets. The histone deacetylase Sirtuin 1 (SIRT1) inhibits adipocyte differentiation by targeting the transcription factor peroxisome proliferator activated-receptor gamma (PPARγ).
Methods
To assess the specific role of SIRT1 in adipocytes, we generated Sirt1 adipocyte-specific knockout mice (ATKO) driven by aP2 promoter onto C57BL/6 background. Sirt1flx/flx aP2Cre+ (ATKO) and Sirt1flx/flx aP2Cre- (WT) mice were fed high-fat diet for 5 weeks (short-term) or 15 weeks (chronic-term). Metabolic studies were combined with gene expression analysis and phosphorylation/acetylation patterns in adipose tissue.
Results
On standard chow, ATKO mice exhibit low-grade chronic inflammation in adipose tissue, along with glucose intolerance and insulin resistance compared with control fed …
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R Mayoral, O Osborn, J McNelis, AM Johnson… - Molecular metabolism, 2015