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This study was undertaken to evaluate antioxidant property, cytotoxicity and antimicrobial activity of Lasia spinosa leaves. Ethyl acetate soluble partitionate of the methanolic extract of the plant material showed significant antioxidant property using DPPH (1, 1-diphenyl-2-pecrylhydrazyl) assay with IC50 value of 16.42 µg/ml. In the brine shrimp lethality bioassay, LC50 values obtained from the best fit line slope were 0.544, 3.181, and 4.096 µg/mL for standard (vincristine sulphate), dichloromethane and ethylacetate soluble partitionates of the methanolic extract respectively. The leaves extracts showed mild antimicrobial property.