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The first successful attempt to produce transgenic tobacco cell suspensions expressing a modified version of amarantin, through direct gene transfer using particle gun is described. The physical parameters for transformation were optimized using the gene encoding β-glucuronidase (gusA) as the reporter gene. Statistical analysis revealed that helium pressure, target distance, and osmotic pretreatment had significant influence on transformation efficiency. Of the different variables evaluated, a combination of sorbitol 0.5 M pretreatment, shooting distance of 7 cm and firing pressure of 900 psi were found ideal. Selection of putative transgenic calli was done on Hygromicine-selection medium. Presence of the transgene was confirmed using histological glucuronidase assay and PCR analysis. Accumulation and stable expression of the recombinant amarantin was detected using Western blot analysis …