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Correct diagnosis of pneumocystosis pneumonia depends on suitable sampling procedure and staining method. The aim of this study was comparison two staining methods “Giemsa and GMS” in three collected samples from studied animal model “oral swab, BAL and lung homogenate”.

Twenty two female rats of two month-ages (Sprague-Dawley) with 150-200 grams of weight were used. Methyl prednizolone acetate was used to stimulate pneumocystosis. Oral swab (OS), broncho-alveolar lavage (BAL) and lung homogenate (LH) were collected during studied weeks from control and test groups. All samples were stained with Giemsa and GMS (Gomori's Methenamine Silver). These specimens were classified in four stages based on 35 microscopic fields (100X) for counting of cyst numbers, tested by two staining methods for common specificity such as number of cysts, required time for observations, cost, and possibility of improper diagnosis.