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The present work describes a novel attempt to produce myco-granules targeted for multiple industrial applications including dye and heavy metal sequestration and xylanase production. Three low cost, locally available substrates FS1 (rice flour), FS2 (maize flour) and FS3 (wheat flour) were characterized and used for producing three myco-granules MG1, MG2 and MG3, respectively. Spore load of freshly prepared myco-granules and after 6 months of storage at 30 °C was estimated. MG1 exhibited least decrease (32%) in number of spores as compared to MG2 (40%) and MG3 (87%). Shelf-life studies conducted for different myco-granules stored at 4 °C, 30 °C and ambient temperature conditions show unaltered biomass production for the initial three months which rules out the need for controlled temperature conditions during storage. When stored at 30 °C, MG1 could remove 83% Acid Navy Blue dye, 57% Cu(II …