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The analysis of stable isotopes on fossil mammals has become a widely used tool for understanding the paleoecology and paleodiet of these organisms. In this work, we study the stable isotope composition of collagen (δ¹³C and δ¹⁵N) and bioapatite (δ¹³C and δ¹⁸O) of fossil bones from Arroyo del Vizcaíno, a fossiliferous site in southern Uruguay dated to ∼32 ka cal BP. Fourteen taxa were analyzed: the ground sloths Lestodon armatus, Glossotherium robustum, Mylodon darwinii, Valgipes bucklandi and Nothrotheriops sp.; the glyptodonts Glyptodon reticulatus, Panochthus tuberculatus, and Doedicurus clavicaudatus; the equids Hippidion principale and Equus neogeus; the proboscidean Notiomastodon platensis; the notoungulate Toxodon platensis; the saber-tooth felid Smilodon populator; and an indeterminate cervid. In general, the percentage of nitrogen in collagen and the C:N ratio were within the limits …