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Structured lipids resembling human milk fat (HMF) enriched with omega-3 fatty acids were synthesized by enzymatic acidolysis reactions between tripalmitin, hazelnut oil fatty acids (FA), and omega-3 FA concentrate. Response surface methodology was used to model and optimize the incorporation of omega-3 FA and oleic acid into tripalmitin, in hexane, using immobilized sn-1,3-specific lipase, Lipozyme RM IM. The three factors chosen were substrate molar ratio, reaction temperature, and reaction time. Good quadratic models were obtained for the incorporation of eicosapentaenoic acid (EPA) plus docosahexaenoic acid (DHA) (response 1) and oleic acid (response 2) by multiple regression and backward elimination. The determination coefficient (R ²) value for the models was 0.95. The adjusted R ² values were 0.91 and 0.92 for responses 1 and 2, respectively. The optimal conditions generated from the …