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Evaluation of meat volatiles is an increasing concern due to the development studies for spoilage sensors in order to inform consumers if meat spoiled or not. Since the reliable measuring of volatiles in headspace is important for designing a sensor, preconcentration techniques for meat matrix should be investigated. This study describes the effect of three sample amounts: total volume ratio (loading factors) with 0.025, 0.05, and 0.10 kg/L applied in two preconcentration sampling techniques: solid phase microextraction (SPME) and gas flushing on Tenax with thermal desorption to explain the interactions and relationship between sample amount and extracted analytes for spoiled beef striploin. The ratio of the sample amount of beef meat in the chamber and the total volume of chamber is called as “loading factor”. The results showed that the volatile profiles did not vary significantly, but the levels were …