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The Chinese dwarf cherry (Cerasus humilis; 2n = 16) has been increasingly prized for its colorful fruits, high economic values, nutraceutical benefits, and wide adaptability for diverse soil and environmental conditions. Polyploid induction is an efficient way to develop new selections and cultivars of horticultural crops. In this study, successful induction of tetraploids (2n = 32) in C. humilis (cultivar: ‘Nongda 7’) was achieved and the tetraploid plants were subsequently characterized. The highest mutagenesis rate reached in our study was 30.0 % with 80 mg/L colchicine added to the solid Murashige and Skoog (MS) media for both the embryo and stem tip. In liquid media, the highest mutagenesis rate achieved was 16.7 % with the addition of 60 mg/L colchicine. Systematic examination of the induced tetraploid mutants, significant morphological differences were observed in leaves, stems, and roots. Tetraploid mutants …