作者
Katrin Streckfuss-Bömeke, Frieder Wolf, Azadeh Azizian, Michael Stauske, Malte Tiburcy, Stefan Wagner, Daniela Hübscher, Ralf Dressel, Simin Chen, Jörg Jende, Gerald Wulf, Verena Lorenz, Michael P Schön, Lars S Maier, Wolfram H Zimmermann, Gerd Hasenfuss, Kaomei Guan
发表日期
2013/9/1
期刊
European heart journal
卷号
34
期号
33
页码范围
2618-2629
出版商
Oxford University Press
简介
Aims
Induced pluripotent stem cells (iPSCs) provide a unique opportunity for the generation of patient-specific cells for use in disease modelling, drug screening, and regenerative medicine. The aim of this study was to compare human-induced pluripotent stem cells (hiPSCs) derived from different somatic cell sources regarding their generation efficiency and cardiac differentiation potential, and functionalities of cardiomyocytes.
Methods and results
We generated hiPSCs from hair keratinocytes, bone marrow mesenchymal stem cells (MSCs), and skin fibroblasts by using two different virus systems. We show that MSCs and fibroblasts are more easily reprogrammed than keratinocytes. This corresponds to higher methylation levels of minimal promoter regions of the OCT4 and NANOG genes in keratinocytes than in MSCs and fibroblasts. The success rate and reprogramming …
学术搜索中的文章
K Streckfuss-Bömeke, F Wolf, A Azizian, M Stauske… - European heart journal, 2013