作者
Eiji Osaka, Andrew D Kelly, Dimitrios Spentzos, Edwin Choy, Xiaoqian Yang, Jacson K Shen, Pei Yang, Henry J Mankin, Francis J Hornicek, Zhenfeng Duan
发表日期
2015/4/4
期刊
Oncotarget
卷号
6
期号
11
页码范围
9125
出版商
Impact Journals, LLC
简介
Background
Chordoma pathogenesis remains poorly understood. In this study, we aimed to evaluate the relationships between microRNA-155 (miR-155) expression and the clinicopathological features of chordoma patients, and to evaluate the functional role of miR-155 in chordoma.
Methods
The miRNA expression profiles were analyzed using miRNA microarray assays. Regulatory activity of miR-155 was assessed using bioinformatic tools. miR-155 expression levels were validated by reverse transcription-polymerase chain reaction. The relationships between miR-155 expression and the clinicopathological features of chordoma patients were analyzed. Proliferative, migratory and invasive activities were assessed by MTT, wound healing, and Matrigel invasion assays, respectively.
Results
The miRNA microarray assay revealed miR-155 to be highly expressed and biologically active in chordoma. miR-155 …
学术搜索中的文章
E Osaka, AD Kelly, D Spentzos, E Choy, X Yang… - Oncotarget, 2015