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Good transparency, antimicrobial, physical, and tensile properties of the biodegradable film can be necessary for food packaging. The aim of this study is to characterize these properties of the poly(vinyl alcohol) (PVA)/ginger nanofiber (GF) bionanocomposite film. This nanofiber of 0.21, 0.31 and 0.41 g in suspensions, was mixed with PVA gel using ultrasonication. After addition of ginger nanofibers, the bionanocomposite film shows antibacterial activity but does not have antifungi activity. Increasing the nanofiber into PVA increases significantly in tensile properties, water vapour impermeability, and moisture resistance. Tensile strength, the temperature at maximum film decomposition, and moisture resistance (after 8 h) of the 0.41 g ginger nanofiber reinforced film were 44.2 MPa (increased by 65.6%), 349.4 °C (increased by 7%), and 6.1% (decreased by 18.7%), respectively compared to pure PVA. With this …