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Iron is vital for bacteria, as it plays a central role in energy production, intermediate metabolism, DNA synthesis, nitrogen fixation etc. to survive in host system, Bacteria primarily secrete siderophores that specifically chelate iron with high affinity and actively transport this ferric siderophore complex inside cell. Siderophore production and ferric siderophore acquisition are frequently associated with microbial infections. Moreover, pathogenic bacteria obtain iron with TonB dependent ferric siderophore transport systems. For example, the outer membrane protein IutA actively transports the iron complex ferric aerobactin (FeAero), and the inner membrane protein TonB provides the energy for this uptake reaction. I created fluorescent sensors that monitor high affinity binding reactions, and used them to detect, discriminate and quantify ferric siderophores, as either isolated iron complexes or in complex mixture of …