作者
Ulhas Sopanrao Kadam, Rahul Mahadev Shelake, Rahul L Chavhan, Penna Suprasanna
发表日期
2018/10/1
来源
Plant Physiology and Biochemistry
卷号
131
页码范围
22-30
出版商
Elsevier Masson
简介
Genome editing (GE) tools ensure targeted mutagenesis and sequence-specific modification in plants using a wide resource of customized endonucleases; namely, zinc-finger nucleases (ZFNs), and transcription activator-like effector nucleases (TALENs), and the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) system. Among these, in recent times CRISPR/Cas9 has been widely used in functional genomics and plant genetic modification. A significant concern in the application of GE tools is the occurrence of ‘off-target’ activity and induced mutations, which may impede functional analysis and gene activity studies. Moreover, the ‘off-target’ activity results in either not reported or unknown, difficult to detect, produce non-quantifiable cellular signaling and physiological effects. In the past few years, several experimental methods have been developed to identify …
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US Kadam, RM Shelake, RL Chavhan, P Suprasanna - Plant Physiology and Biochemistry, 2018