作者
Glaucia Mendes Souza, Marie-Anne Van Sluys, Carolina Gimiliani Lembke, Hayan Lee, Gabriel Rodrigues Alves Margarido, Carlos Takeshi Hotta, Jonas Weissmann Gaiarsa, Augusto Lima Diniz, Mauro de Medeiros Oliveira, Sávio de Siqueira Ferreira, Milton Yutaka Nishiyama Jr, Felipe Ten-Caten, Geovani Tolfo Ragagnin, Pablo de Morais Andrade, Robson Francisco De Souza, Gianlucca Gonçalves Nicastro, Ravi Pandya, Changsoo Kim, Hui Guo, Alan Mitchell Durham, Monalisa Sampaio Carneiro, Jisen Zhang, Xingtan Zhang, Qing Zhang, Ray Ming, Michael C Schatz, Bob Davidson, Andrew H Paterson, David Heckerman
发表日期
2019/12
期刊
GigaScience
卷号
8
期号
12
页码范围
giz129
出版商
Oxford University Press
简介
Background
Sugarcane cultivars are polyploid interspecific hybrids of giant genomes, typically with 10–13 sets of chromosomes from 2 Saccharum species. The ploidy, hybridity, and size of the genome, estimated to have >10 Gb, pose a challenge for sequencing.
Results
Here we present a gene space assembly of SP80-3280, including 373,869 putative genes and their potential regulatory regions. The alignment of single-copy genes in diploid grasses to the putative genes indicates that we could resolve 2–6 (up to 15) putative homo(eo)logs that are 99.1% identical within their coding sequences. Dissimilarities increase in their regulatory regions, and gene promoter analysis shows differences in regulatory elements within gene families that are expressed in a species-specific manner. We exemplify these differences for sucrose synthase (SuSy) and phenylalanine ammonia …
学术搜索中的文章
GM Souza, MA Van Sluys, CG Lembke, H Lee… - GigaScience, 2019