查看文章

The anti‐HLA antibody can be detected using either a complement‐dependent lymphocytotoxicity (CDC) assay or a flow cytometry crossmatch (FCXM) in renal transplantation. Discordant results are often obtained because the two methods detect different reaction phases between the donor lymphocytes and the recipient sera. This study was intended to confirm that antibody binding and cytotoxicity to the lymphocytes can be detected simultaneously in a single FCXM assay, cytotoxic FCXM.

In the cytotoxic FCXM, the antibody binding to the lymphocytes was measured using anti‐IgG‐FITC, and the cytotoxicity using 7‐aminoactinomycin D (7‐AAD) after adding complement. For an evaluation of two test parameters, the cytotoxicity test moiety (dead‐cell percentage) was compared with the anti‐human globulin (AHG)‐CDC, and the antibody‐binding test moiety (sample/control fluorescence …