作者
Mette J Nielsen, Anders F Nedergaard, Shu Sun, Sanne S Veidal, Lise Larsen, Qinlong Zheng, Charlotte Suetta, Kim Henriksen, Claus Christiansen, Morten A Karsdal, Diana J Leeming
发表日期
2013
期刊
American journal of translational research
卷号
5
期号
3
页码范围
303
出版商
e-Century Publishing Corporation
简介
Aim
The present study describes the assessment of true formation of type III collagen in different pathologies using a neo-epitope specific competitive Enzyme-linked immunosorbent assay (ELISA) towards the N-terminal propeptide of type III collagen (PRO-C3). Methods
The monoclonal antibody was raised against the N-protease mediated cleavage site of the N-terminal propeptide of type III collagen and a competitive ELISA was developed using the selected antibody. The assay was evaluated in relation to neo-epitope specificity, technical performance, and as a marker for liver fibrosis and muscle mass using the rat carbon tetrachloride (CCl 4) model and a study of immobilization induced muscle loss in humans, respectively. Results
The ELISA was neo-epitope specific, technically stable and can be assessed in serum and plasma samples. In the CCl 4 liver fibrosis model it was observed that serum PRO-C3 …学术搜索中的文章
MJ Nielsen, AF Nedergaard, S Sun, SS Veidal… - American journal of translational research, 2013