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Organophosphorus pesticides (OPs) are used in crops to eradicate pests, but are of concern to the public, attributable to their potential toxicity to human health. In the present study, a biodegradable composite film containing citrate-stabilized gold nanoparticles (AuNPs) was developed to detect pesticides. The proposed composite film has distinctive structural and functional characteristics as a colorimetric recognition indicator for detecting OPs (malathion, fenthion, dimethoate, and chlorpyrifos). The developed film was morphologically and spectroscopically characterized through electron microscopy and UV–Vis spectrometry, respectively. The spherical-shaped and monodispersed AuNPs were obtained with an average particle size of 20 nm. The aggregation of the AuNPs composite film in the presence of pesticides led to a change in color from red to blueish purple, which could be visually observed on the …