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The Internet of Things (IoT) brings a multidisciplinary revolution in several application areas. However, security and privacy concerns are undermining a reliable and resilient broad-scale deployment of IoT-enabled critical infrastructures (IoT-CIs). To fill this gap, this paper proposes a comprehensive architectural design that captures the main security and privacy challenges related to cyber-physical systems and IoT-CIs. The architecture is devised to empower IoT systems and networks to make autonomous security decisions through the usage of novel technologies such as software defined networking and network function virtualization, as well as endowing them with intelligent and dynamic security reaction capabilities by relying on monitoring methodologies and cyber-situational tools. The architecture has been successfully implemented and evaluated in the scope of ANASTACIA H2020 EU research project.