作者
F Saraee, F Shekari, A Moini, M Sadeghi, P Ghaznavi, A Nazari, A Ghaheri, M Totonchi, P Eftekhari-Yazdi
发表日期
2022/7/1
期刊
Human Reproduction
卷号
37
期号
Supplement_1
页码范围
deac107. 365
出版商
Oxford University Press
简介
Study question
What is the suitable method for isolating extracellular vesicles (EVs) from a small volume of Uterine Fluid Lavage (UFL)?
Summary answer
It seems that the qEV column can help in faster, precise, and gentle isolation of EVs from small amounts of UFL.
What is known already
Several studies have discovered that existing EVs in the UFL, which are informative carriers, reflect cytological and molecular conditions of the uterine environment. The relative amounts of the EV components are different in various physiological situations. So, they are the ideal source for precise molecular analysis of the uterine condition to identify the Window of Implantation (WOI) for appropriate embryo transfer (ET) timing. There is limited information describing the most efficient method for EV isolation from a low-volume sample of biological fluids. In particular, EV …