作者
Saman Firoozi, Sara Pahlavan, Mohammad-Hossein Ghanian, Shahram Rabbani, Maryam Barekat, Abdoreza Nazari, Mohammad Pakzad, Faezeh Shekari, Seyedeh-Nafiseh Hassani, Fariba Moslem, Fatemeh Nobakht Lahrood, Mansoureh Soleimani, Hossein Baharvand
发表日期
2020/4/16
期刊
Biochemical and Biophysical Research Communications
卷号
524
期号
4
页码范围
903-909
出版商
Academic Press
简介
Purpose
The aim of this study was to investigate the cardiac repair effect of human bone marrow mesenchymal stromal cells-derived extracellular vesicles (MSC-EVs) after intramyocardial injection in free form or encapsulated within a self-assembling peptide hydrogel modified with SDKP motif, in a rat model of myocardial infarction (MI).
Methods
MSC-EVs were isolated by ultracentrifuge and characterized for physical parameters and surface proteins. Furthermore, cellular uptake and cardioprotective effects of MSC-EVs were evaluated in vitro using neonatal mouse cardiomyocytes (NMCMs). In vivo effects of MSC-EVs on cardiac repair were studied in rat MI model by comparing the vehicle group (injected with PBS), EV group (injected with MSC-EVs) and Gel + EV group (injected with MSC-EVs encapsulated in (RADA)4-SDKP hydrogel) with respect to cardiac function and fibrotic area using echocardiography and …
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S Firoozi, S Pahlavan, MH Ghanian, S Rabbani… - Biochemical and Biophysical Research …, 2020