作者
Nasim Eslami, Khadijeh Bahrehbar, Fereshteh Esfandiari, Faezeh Shekari, Seyedeh-Nafiseh Hassani, Abdoreza Nazari, Mohammad Pakzad, Hossein Baharvand
发表日期
2023/5/15
期刊
Life Sciences
卷号
321
页码范围
121536
出版商
Pergamon
简介
Aims
Some studies have shown that mesenchymal stem cells (MSCs) and their derived extracellular vesicles (MSC-EVs) can restore ovarian function in premature ovarian failure (POF), however, concerns about their efficacy are attributed to the heterogeneity of the cell populations and EVs. Here, we assessed the therapeutic potential of a homogeneous population of clonal MSCs (cMSCs) and their EVs subpopulations in a mouse model of POF.
Main methods
Granulosa cells were treated with cyclophosphamide (Cy) in the absence or presence of cMSCs, or cMSCs-derived EV subpopulations (EV20K and EV110K, isolated by high-speed centrifugation and differential ultracentrifugation, respectively). In addition, POF mice were treated with cMSCs, EV20K and/or EV110K.
Key findings
cMSC and both EV types protected granulosa cells from Cy-induced damage. Calcein-EVs were detected in the ovaries. Moreover …
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