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This study investigated the change in 1000‐m simulated rowing performance in two matched groups of 19 competitive rowers following a 5‐day period of supplementation with placebo (CON group) or creatine at a dose equivalent to 0.25 g creatine monohydrate per kilogram of body mass (BM) (EXP group). Creatine uptake was calculated from the difference between the amount fed and the amount recovered in urine during each 24‐h period of supplementation. Total creatine uptake for the EXP group over the 5‐day period of supplementation averaged 34.9 ± 10.9 g (range 20.1–54.9 g), which equated to 3.54 ± 0.93 mmol kg BM^‐1. The estimated creatine uptake into muscle was 38.1 ± 10.0 (range 22.6–56.6) mmol kg dry weight^‐1 for these subjects. After supplementation with placebo, the CON group showed no change in 1000‐m rowing performance (214.0 ± 30.9 vs 214.1 ± 31.5 s; P = 0.88). Of these subjects, 7 …